Event Title

Effects of Increased Salinity on the Expression of HSP70 Protein in Adult Stream-Dwelling Mussels Elliptio complanata

Presenter Information

Kyle Farrow, Biology

Faculty Sponsor(s)

Brigid O'Donnell

Location

Hartman Union Building Courtroom

Presentation Type

Event

Start Date

4-28-2017 2:00 PM

End Date

4-28-2017 3:00 PM

Abstract

In New Hampshire, road salt is frequently used for reducing ice on roads, and during periods of snowmelt in spring, salt runoff to streams varies depending on frequency of road use. Road salt can increase salinity in these streams and negatively impact the individual organisms and communities inhabiting these sites. Native freshwater mussels Elliptio complanta, act as ecosystem engineers for bioturbulation, water column filtering, and denitrification. The objective of my research is to measure the impact of salinity on the expression of heat shock protein 70 (HSP70) in Elliptio complanta. Mussels were collected from the Merrimack River, acclimated in lab, and exposed to acute (4h) or chronic (3d) NaCl concentrations at 0.5ppt, 1ppt, 2ppt and 3ppt. HSP70 expression was measured by collecting gill tissue, extracting total proteins, and Western blotting to probe for HSP70 abundance using a horseradish peroxidase conjugated secondary antibody. HSP70 abundance in mussels exposed to NaCl treatments was not significantly different from untreated individuals (n = 8; one-way ANOVA P > 0.05 ). Future work includes increasing NaCl concentrations to further understand the impact of NaCl on HSP70 expression and to investigate other stress proteins that might be used as potential biomarkers for salt stress.

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Apr 28th, 2:00 PM Apr 28th, 3:00 PM

Effects of Increased Salinity on the Expression of HSP70 Protein in Adult Stream-Dwelling Mussels Elliptio complanata

Hartman Union Building Courtroom

In New Hampshire, road salt is frequently used for reducing ice on roads, and during periods of snowmelt in spring, salt runoff to streams varies depending on frequency of road use. Road salt can increase salinity in these streams and negatively impact the individual organisms and communities inhabiting these sites. Native freshwater mussels Elliptio complanta, act as ecosystem engineers for bioturbulation, water column filtering, and denitrification. The objective of my research is to measure the impact of salinity on the expression of heat shock protein 70 (HSP70) in Elliptio complanta. Mussels were collected from the Merrimack River, acclimated in lab, and exposed to acute (4h) or chronic (3d) NaCl concentrations at 0.5ppt, 1ppt, 2ppt and 3ppt. HSP70 expression was measured by collecting gill tissue, extracting total proteins, and Western blotting to probe for HSP70 abundance using a horseradish peroxidase conjugated secondary antibody. HSP70 abundance in mussels exposed to NaCl treatments was not significantly different from untreated individuals (n = 8; one-way ANOVA P > 0.05 ). Future work includes increasing NaCl concentrations to further understand the impact of NaCl on HSP70 expression and to investigate other stress proteins that might be used as potential biomarkers for salt stress.