Event Title

Daf-2 Gene Silencing and the Response of C. elegans to Oxidative Stress

Presenter Information

Christopher Cox, Biology

Faculty Sponsor(s)

Susan Swope

Location

Hartman Union Building Courtroom

Presentation Type

Event

Start Date

5-3-2018 2:00 PM

End Date

5-3-2018 3:00 PM

Abstract

Caenorhabditis elegans and other nematodes are ectothermic organisms that primarily rely on the environment for temperature regulation. The daf-2 gene has many different functions in C. elegans, including stress resistance to high temperature, oxidative stress, and the capability of entering the developmentally arrested larval state. The daf-2 gene as also been found to regulate longevity in C. elegans. The daf-2 gene is orthologous to IGF1-R in humans, which has been found to be greatly overexpressed in cancerous tissue. PCR was conducted to isolate the daf-2 gene, and the amplicon was inserted into the RNAi feeding vector, which was used to transform E. coli intro a feeding strand for the C. elegans. A juglone sensitivity assay will be conducted on the organisms in order to determine the effects of oxidative stress on daf-2 knockout worms. We predict that the mutant worms will have a shorter lifespan and higher mortality rates compared to the wild type organisms.

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May 3rd, 2:00 PM May 3rd, 3:00 PM

Daf-2 Gene Silencing and the Response of C. elegans to Oxidative Stress

Hartman Union Building Courtroom

Caenorhabditis elegans and other nematodes are ectothermic organisms that primarily rely on the environment for temperature regulation. The daf-2 gene has many different functions in C. elegans, including stress resistance to high temperature, oxidative stress, and the capability of entering the developmentally arrested larval state. The daf-2 gene as also been found to regulate longevity in C. elegans. The daf-2 gene is orthologous to IGF1-R in humans, which has been found to be greatly overexpressed in cancerous tissue. PCR was conducted to isolate the daf-2 gene, and the amplicon was inserted into the RNAi feeding vector, which was used to transform E. coli intro a feeding strand for the C. elegans. A juglone sensitivity assay will be conducted on the organisms in order to determine the effects of oxidative stress on daf-2 knockout worms. We predict that the mutant worms will have a shorter lifespan and higher mortality rates compared to the wild type organisms.