Event Title

The Effects of Sambucus nigra Extract on Paralysis Rate of Caenorhabditis elegans

Faculty Sponsor(s)

Susan Swope

Location

Hartman Union Building Courtroom

Presentation Type

Event

Start Date

5-3-2018 3:00 PM

End Date

5-3-2018 4:00 PM

Abstract

A growing body of evidence suggests that maintenance of proteostasis is necessary to health. Failure to maintain protein quality results in aggregation of toxic oligomers leading to progressive degeneration. CL4176 is a strain of C. elegans genetically engineered to overexpress amyloid beta (Aβ) protein, resulting in accumulation of the Aβ-oligomer and leading to paralysis. Subjecting C. elegans to treatments that mitigate the accumulation of Aβ-oligomer may promote prolongevity of the worms by aiding in the maintenance of the proteome. The plant Sambucus nigra has high concentrations of anthocyanin, a bioactive compound that may promote the maintenance of proteostasis. Four different populations of CL4176 will be exposed to varying concentrations of Sambucus nigra extract. One population will be exposed to a control containing no active compound, and another will be exposed to a positive control of caffeine. The worms will be scored according to standard lifetime assay procedure. Prolonged lifespan of the worms may suggest protective strategies of the extract to slow proteostasis decline during nematode aging.

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May 3rd, 3:00 PM May 3rd, 4:00 PM

The Effects of Sambucus nigra Extract on Paralysis Rate of Caenorhabditis elegans

Hartman Union Building Courtroom

A growing body of evidence suggests that maintenance of proteostasis is necessary to health. Failure to maintain protein quality results in aggregation of toxic oligomers leading to progressive degeneration. CL4176 is a strain of C. elegans genetically engineered to overexpress amyloid beta (Aβ) protein, resulting in accumulation of the Aβ-oligomer and leading to paralysis. Subjecting C. elegans to treatments that mitigate the accumulation of Aβ-oligomer may promote prolongevity of the worms by aiding in the maintenance of the proteome. The plant Sambucus nigra has high concentrations of anthocyanin, a bioactive compound that may promote the maintenance of proteostasis. Four different populations of CL4176 will be exposed to varying concentrations of Sambucus nigra extract. One population will be exposed to a control containing no active compound, and another will be exposed to a positive control of caffeine. The worms will be scored according to standard lifetime assay procedure. Prolonged lifespan of the worms may suggest protective strategies of the extract to slow proteostasis decline during nematode aging.