Event Title

Characterization of Medicinal Compounds Found in Various Species of Basil

Faculty Sponsor(s)

Anil Waghe

Abstract

Basil, a popular culinary and medicinal herb, is cultivated in several countries resulting in a distinct flavor variation among the cultivars. This flavor variation, specifically among Thai basil (Ocimum basilicum var. thyrsiflorum), Italian (sweet) basil (Ocimum basilicum), and Indian basil (Ocimum sanctum), prompted us to explore the variations in the medicinal compounds of the three different species. Dried Basil species collected from various parts of the world were extracted using the Soxhlet apparatus. Active ingredients methyl eugenol, eugenol, methyl chavicol, and linalool were identified in the various species using Thin Layer Chromatography (TLC) and Gas Chromatography/Mass Spectrometry (GC/MS).  The TLC analysis showed separation of 2-4 compounds for various species, with Rf values ranging from 0.244 to 0.686.  The antimicrobial properties were tested against bacillus cereus using the Kirby-Bauer disc diffusion technique with negative and positive controls of ethanol (solvent) and tetracycline, respectively. The results were compared between the different species and their corresponding chemical constituents.

Location

Hartman Union Building Courtroom

Start Date

5-2-2019 4:00 PM

End Date

5-2-2019 5:00 PM

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May 2nd, 4:00 PM May 2nd, 5:00 PM

Characterization of Medicinal Compounds Found in Various Species of Basil

Hartman Union Building Courtroom

Basil, a popular culinary and medicinal herb, is cultivated in several countries resulting in a distinct flavor variation among the cultivars. This flavor variation, specifically among Thai basil (Ocimum basilicum var. thyrsiflorum), Italian (sweet) basil (Ocimum basilicum), and Indian basil (Ocimum sanctum), prompted us to explore the variations in the medicinal compounds of the three different species. Dried Basil species collected from various parts of the world were extracted using the Soxhlet apparatus. Active ingredients methyl eugenol, eugenol, methyl chavicol, and linalool were identified in the various species using Thin Layer Chromatography (TLC) and Gas Chromatography/Mass Spectrometry (GC/MS).  The TLC analysis showed separation of 2-4 compounds for various species, with Rf values ranging from 0.244 to 0.686.  The antimicrobial properties were tested against bacillus cereus using the Kirby-Bauer disc diffusion technique with negative and positive controls of ethanol (solvent) and tetracycline, respectively. The results were compared between the different species and their corresponding chemical constituents.