Event Title

Effects of Elderberry Extract on the Insulin/ IGF-1 (IIS) Pathway of Caenorhabditis elegans

Faculty Sponsor(s)

Susan Swope

Abstract

Proteostasis is an essential aspect of protein regulation, and the inability to maintain the proteome can lead to many neurodegenerative diseases. Caenorhabditis elegans, CL4176 is a transgenic strain designed to produce β-amyloid42 (Aβ), disrupt proteostasis, and provide insight into age-related diseases. Treatments with elderberry (S. nigra) extract (EBE) results in a decrease in Aβ toxicity and extension of life span. To explore the involvement of the insulin/ IGF-1 (IIS) metabolic pathway, we knocked down the age-1, daf-2, daf-16, and hsf-1 genes. We then confirmed knock downs with RT-PCR, and found that EBE affects lifespan expansion primarily by hsf-1. These results are used to confirm that EBE extends lifespan through the IIS pathway, and will contribute to further study on potential benefits in humans.

Location

Hartman Union Building Courtroom

Start Date

5-2-2019 2:00 PM

End Date

5-2-2019 3:00 PM

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May 2nd, 2:00 PM May 2nd, 3:00 PM

Effects of Elderberry Extract on the Insulin/ IGF-1 (IIS) Pathway of Caenorhabditis elegans

Hartman Union Building Courtroom

Proteostasis is an essential aspect of protein regulation, and the inability to maintain the proteome can lead to many neurodegenerative diseases. Caenorhabditis elegans, CL4176 is a transgenic strain designed to produce β-amyloid42 (Aβ), disrupt proteostasis, and provide insight into age-related diseases. Treatments with elderberry (S. nigra) extract (EBE) results in a decrease in Aβ toxicity and extension of life span. To explore the involvement of the insulin/ IGF-1 (IIS) metabolic pathway, we knocked down the age-1, daf-2, daf-16, and hsf-1 genes. We then confirmed knock downs with RT-PCR, and found that EBE affects lifespan expansion primarily by hsf-1. These results are used to confirm that EBE extends lifespan through the IIS pathway, and will contribute to further study on potential benefits in humans.